A man who says he is an Iranian nuclear scientist claims to have escaped after being abducted by US agents.
In the video, the man says: "A few minutes ago I succeeded in escaping US security agents"
Mr Amiri disappeared a year ago while undertaking the Hajj in Saudi Arabia.
Two videos purportedly showing him surfaced three weeks ago. One said he had been kidnapped, the other that he was living freely in Arizona.
The US has strenuously denied abducting him, but ABC News reported in March that Mr Amiri had defected and was helping the CIA compile intelligence on Iran's controversial nuclear weapons programme.
The state department has refused to say whether he is in the US.
'Not free' In the new video, broadcast on Tuesday, a man claiming to be the missing scientist says: "I, Shahram Amiri, am a national of the Islamic Republic of Iran and a few minutes ago I succeeded in escaping US security agents in Virginia.
"Presently, I am producing this video in a safe place. I could be re-arrested at any time."
Two videos supposedly showing Shahram Amiri emerged on 8 June
"The second video which was published on YouTube by the US government, where I have said that I am free and want to continue my education here, is not true and is a complete fabrication.
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"I am not free here and I am not permitted to contact my family. If something happens and I do not return home alive, the US government will be responsible."Shahram Amiri 29 June broadcast 8 June video transcriptsI was not prepared to betray my country under any kind of threats or bribery by the US government
He finishes the video by urging Iranian officials and human rights organisations to "put pressure on the US government for my release and return".
"I was not prepared to betray my country under any kind of threats or bribery by the US government," he adds.
A US official told the AFP news agency the allegations were "ludicrous".
Iranian media have said Mr Amiri worked as a researcher at a university in Tehran, but some reports say he worked for the country's atomic energy organisation and had in-depth knowledge of its nuclear programme.
BBC
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